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标题：Applications of MTT Assay to Primary Cultured Rat Hepatocytes
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作者：Takeru FUJII ; Hun HA ; Hideakira YOKOYAMA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1995
卷号：18
期号：10
页码：1446-1449
DOI：10.1248/bpb.18.1446
出版社：The Pharmaceutical Society of Japan
摘要：We investigated the optimal conditions for the application of the MTT assay to determine the viability of cell in primary cultures of rat hepatocytes. The optimal conditions were found to be : inoculation at cell density of 1.31×10⁵ cells/cm² ; concentration of 3-(4, 5-dimethylthiazoyl-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) 0.12mM ; and MTT incorporation time, 2 h or more. We also found that insulin and glucocorticoid, which are usually added to the medium to maintain cell function, had no effect on the viability of the primary cultures of rat hepatocytes.
关键词：MTT assay;primary cultured rat hepatocyte;cell density;hormone-free