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  • 标题:QUANTITATIVE EVALUATION OF PASSIVE CUTANEOUS ANAPHYLAXIS (PCA) USING A HAND-HELD SPECTROPHOTOMETER
  • 本地全文:下载
  • 作者:Hiroshi AKIYAMA ; Reiko TESHIMA ; Reiko AKASAKA
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:1996
  • 卷号:19
  • 期号:8
  • 页码:1112-1114
  • DOI:10.1248/bpb.19.1112
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:A hand-held spectrophotometer was used for the quantitative evaluation of PCA caused by anti-TNP-IgE and TNP-BSA. A good relationship existed between the dilution of sera and the value of ΔE*ab (a numerical expression of color) obtained by a hand-held spectrophotometer. In addition, the value of ΔE*ab and the amount of Evans blue measured by the conventional colorimetric method correlated well. Because the method using a hand-held spectrophotometer provides a simple and objective analysis, it appears that the method is suitable as a substitute for the conventional method, which is time-consuming and requires killing animals cruelly.
  • 关键词:passive cutaneous anaphylaxis (PCA);spectrophotometer;rat;immediate-type hypersensitivity;Evans blue
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