文章基本信息

标题：Variable Glyceryl Dinitrate Formation as a Function of Glutathione S-Transferase
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作者：Tomokazu FUJII ; Tetsuo ADACHI ; Yoshiko USAMI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：8
页码：1093-1096
DOI：10.1248/bpb.19.1093
出版社：The Pharmaceutical Society of Japan
摘要：Nitroglycerin (GTN) has been used as the drug of choice in the treatment of angina pectoris. It has been shown that some glutathione S-transferases (GSTs) catalyze the metabolic conversion from GTN to glyceryl dinitrates (GDNs). In this study, we examined the substrate specificity of GSTs for GTN. Alpha and mu GSTs were isolated from porcine liver and intestinal mucosa by means of CM-cellulose and glutathione-affinity column chromatography. Mu GSTs degraded GTN time-dependently and formed 1, 3-GDN in preference to 1, 2-GDN at a ratio (1, 2-GDN/1, 3-GDN) of 0.61, whereas alpha GSTs formed twice as much 1, 2-GDN as 1, 3-GDN. These results showed that two GST families participate in the metabolic conversion of GTN at different hydrolyzing portions of the nitrogroups.
关键词：glutathione S-transferase;nitroglycerin;glyceryl dinitrate