文章基本信息

标题：Estrogen Receptor-Mediated Transcriptional Activation by 17β-Estradiol and Its Analogs
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作者：Taiko ODA ; Terumasa TSUCHIYA ; Yoshihiro SATO 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：8
页码：1018-1022
DOI：10.1248/bpb.19.1018
出版社：The Pharmaceutical Society of Japan
摘要：We investigated the effects of natural and synthetic estrogens on estrogen receptor-mediated signal transduction and transcription. To assess estrogen receptor-dependent gene expression, we constructed a reporter plasmid that can express the luciferase gene under the control of the estrogen-responsive element. A transcriptional assay was performed by transfection of the reporter plasmid into estrogen-responsive MCF-7 cells. Transcription was completely dependent on the natural estrogen, 17β-estradiol (E₂), in a dose-dependent manner. Retinoic acids and other steroid hormones had no effect on gene expression. The effect of synthetic estrogens on estrogen receptor-mediated signal transduction was also examined using the specific reporter assay system. A synthetic estrogen, diethylstilbestrol, exhibited higher activity than E₂ at lower concentrations. In addition, transcriptional activities of the (+)- and (-)-enantiomers of indenestrol A, a metabolite of diethylstilbestrol, could be distinguished using the reporter assay system.
关键词：estrogen;diethylstilbestrol;estrogen receptor;MCF-7 cell;signal transduction