文章基本信息

标题：LONG-TERM ADMINISTRATION OF "SHO-SAIKO-TO" INCREASES CYTOCHROME P-450 mRNA LEVEL IN MOUSE LIVER
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作者：keisuke KOJIMA ; Hajime MIZUKAMI ; Takako TAZAWA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1998
卷号：21
期号：4
页码：426-428
DOI：10.1248/bpb.21.426
出版社：The Pharmaceutical Society of Japan
摘要：Simplified differential display of mRNA was applied to isolate and identify genes transcriptionally regulated in mouse liver by sho-saiko-to administration. A cDNA fragment up-regulated by sho-saiko-to was isolated and characterized. cDNA sequencing and subsequent database analysis revealed that the fragment showed significant sequence similarity with mouse testosterone 16-alpha-hydroxylase (cytochrome P-450_16α) cDNA. The increased level of mRNA expression of cytochrome P-450_16α in association with sho-saiko-to administration suggests the molecular mechanism of the chemopreventive effect of sho-saiko-to. This result indicates the usefulness of the mRNA differential display technique to investigate the molecular mechanism of Kampo medicine.
关键词：PCR;differential display;sho-saiko-to;transcriptional regulation;cytochrome P-45016α cDNA