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标题：Determination of Urinary and Serum Pentosidine and Its Application to Elder Patients
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作者：Kazuhiro YOSHIHARA ; Kyoko NAKAMURA ; Manabu KANAI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1998
卷号：21
期号：10
页码：1005-1008
DOI：10.1248/bpb.21.1005
出版社：The Pharmaceutical Society of Japan
摘要：Pentosidine, a fluorescent cross-linking compound, accumulates in extracellular matrix, espcially in the collagen, and is formed by the nonenzymatic process of advanced Maillard reaction. We developed a method of determination of pentosidine and tried to examine its level in urine and serum of elder patients. The method, which involves the hydrolysis of samples, pretreatment using a CF-11 cellulose column and HPLC quantification, resulted in a high recovery (94.3%) of pentosidine with low coefficient of variation (8-10%) of total analysis. Serum and urinary levels of pentosidine in control subjects gradually increased with age. Elder patients with cerebral infarction showed higher levels of serum and urinary pentosidine as compared with those with senile dementia and other geriatric disorders. These results suggest that serious damage to systemic vascular tissues has already occurred in these patients due to glycation.
关键词：pentosidine;urinary excretion level;serum;biomedical marker;HPLC;cerebral infarction