文章基本信息

标题：Determination of Insulin Content in Pancreatic β Cell Line MIN6 Cells by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
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作者：Atsushi TAMURA ; Tsuyoshi AZUMA ; Kayo IKEDA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1998
卷号：21
期号：11
页码：1240-1241
DOI：10.1248/bpb.21.1240
出版社：The Pharmaceutical Society of Japan
摘要：The insulin content in mouse insulinoma MIN6 cells was determined using matrix-assisted laser desorption ionization (MALDI) time-of-flight mass spectrometry (TOF/MS). A mass spectrum of cellular insulin was obtained with a cell burst of MIN6 by hypotonic water. Signal intensities of intracellular insulin were proportional to the number of MIN6 cells. The present method was applied to the determination of intracellular insulin content of MIN6 before and after glucose stimulation.
关键词：MALDI-TOF/MS;MIN6 cells;intracellular insulin content