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  • 标题:DNA Profiling of Acorus calamus Chemotypes Differing in Essential Oil Composition
  • 本地全文:下载
  • 作者:Naoki SUGIMOTO ; Fumiyuki KIUCHI ; Masayuki MIKAGE
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:1999
  • 卷号:22
  • 期号:5
  • 页码:481-485
  • DOI:10.1248/bpb.22.481
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:The phylogenetic relationship of Acorus gramineus and three types of Acorus calamus was analyzed by comparing the 700 bp sequence of a 5S-rRNA gene spacer region. Although there was no intra-specific variation in the essential oil profile of A. gramineus which contained a phenylpropanoid (Z-asarone) as a predominant constituent, A. calamus was classified into two chemotypes : chemotype A in which Z-asarone is a major essential oil constituent and chemotype B which contained sesquiterpenoids predominantly. An intermediate type (M) of these two chemotypes in various ratios was also observable. The NJ tree constructed based on the sequences revealed that A. gramineus was clearly distinguished from any of the chemotypes of A. calamus and that the phylogenetic relationship predicted by the spacer region data correlated well with the essential oil chemotype pattern of A. calamus.
  • 关键词:molecular phylogeny;essential oil chemotype;5S-rRNA gene;nucleotide sequence;Acorus calamus;Acorus gramineus
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