文章基本信息

标题：The Hydroxyl Radical Formation System in Polymorphonuclear Leukocytes
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作者：Naoko KAWAKAMI ; Takao HAYAKAWA ; Shun SHIMOHAMA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1999
卷号：22
期号：10
页码：1034-1037
DOI：10.1248/bpb.22.1034
出版社：The Pharmaceutical Society of Japan
摘要：We studied the hydroxyl radical (OH·)-generating system in polymorphonuclear leukocytes (PMNs). When phenylalanine was incubated with the α, β and γ fractions prepared from pig polymorphonuclear leukocytes (PMNs) and hydrogen peroxide (H₂O₂), significant levels of formation of m- and o-tyrosine were observed in the α and β fractions, but not in the γ fraction. The amount of tyrosine formation per milligram of protein was greater with the β than with the α fraction. Further, when phenylalanine was incubated with α or β fractions with similar myeloperoxidase (MPO) activities in the presence of H₂O₂, tyrosine formation by the β fraction was also more effective. Using the β fraction in which the MPO activity was destroyed by heat treatment, no significant amount of tyrosine was formed. However, with the heat-treated β fraction and MPO preparations from human neutrophils in the presence of H₂O₂, the amount of tyrosine formation increased with the addition of increasing amounts of heat-treated β fraction. Tyrosine formation by the β fraction in the presence of H₂O₂ was significantly reduced by OH·scavengers. The above results suggest the existence of an OH·-generating system in which MPO and H₂O₂ participate in the granules of PMNs and, especially, in specific granules, there may exist some factors that cause more effective OH·generation.
关键词：polymorphonuclear leukocyte;granule;myeloperoxidase;hydroxyl radical