文章基本信息

标题：Inhibition of Listeriolysin O-Induced Hemolysis by Bovine Lactoferrin
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作者：Kohji MORIISHI ; Satoshi INOUE ; Minako KOURA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1999
卷号：22
期号：11
页码：1167-1172
DOI：10.1248/bpb.22.1167
出版社：The Pharmaceutical Society of Japan
摘要：Lactoferrin (LFR) plays an important role in the anti-microbial defense through iron binding, lipopolysaccharide binding and immunomodulation. In this study, we demonstrate that bovine LFR specifically inhibits the hemolytic activity of listeriolysin O (LLO) produced by Listeria monocytogenes. The hemolytic activity of LLO was completely inhibited in the presence of bovine LFR that was highly purified on two cation-exchange columns, whereas that of streptolysin O or perfringolysin O was not inhibited at all. A rabbit anti-LFR antibody canceled this inhibitory activity of bovine LFR. Although human transferrin exhibits 62% amino acid identity with bovine LFR, human apo-transferrin could not inhibit LLO-induced hemolysis. An increase in the concentration of FeCl₃ or the Fe³⁺-saturation of bovine LFR, however, slightly reduced its inhibition of the hemolysis.The inhibitory activity of bovine LFR was dependent on pH, since it was observed under neutral and alkali conditions, but not under acidic conditions. These results suggest that the inhibition of LLO-induced hemolysis by bovine LFR is influenced by pH and iron ions, both of which may lead to conformational changes of LFR.
关键词：lactoferrin;Listeria monocytogenes;listeriolysin O;hemolysin;hemolysis