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标题：Cloning and Characterization of the ddc Homolog Encoding L-2, 4-Diaminobutyrate Decarboxylase in Enterbacter aerogenes
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作者：Shigeo YAMAMOTO ; Nobuo MUTOH ; Daisuke TSUZUKI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2000
卷号：23
期号：5
页码：649-653
DOI：10.1248/bpb.23.649
出版社：The Pharmaceutical Society of Japan
摘要：L-2, 4-Diaminobutyrate decarboxylase (DABA DC) catalyzes the formation of 1, 3-diaminopropane (DAP) from DABA. In the present study, the ddc gene encoding DABA DC from Enterobacter aerogenes ATCC 13048 was cloned and characterized. Determination of the nucleotide sequence revealed an open reading frame of 1470 bp encoding a 53659-Da protein of 490 amino acids, whose deduced NH₂-terminal sequence was identical to that of purified DABA DC from E. aerogenes. The deduced amino acid sequence was highly similar to those of Acinetobacter baumannii and Haemophilus influenzae DABA DCs encoded by the ddc genes. The lysine-307 of the E. aerogenes DABA DC was identified as the pyridoxal 5'-phosphate binding residue by site-directed mutagenesis. Furthermore, PCR analysis revealed the distribution of E. aerogenes ddc homologs in some other species of Enterobacteriaceae. Such a relatively wide occurrence of the ddc homologs implies biological singificance of DABA DC and its product DAP.
关键词：diaminobutyrate decarboxylase;E. aerogenes;gene cloning;Enterobacteriaceae