文章基本信息

标题：Preparation of Fluorescence Labeled Insulins, Sulfobenzoxadiazolyl-insulins, for Fluorescence Immunoassay
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作者：Masahiko NANAMI ; Kiyoshi ZAITSU ; Yosuke OHKURA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：2
页码：99-102
DOI：10.1248/bpb.16.99
出版社：The Pharmaceutical Society of Japan
摘要：The preparation of insulins labeled with a fluorescent moiety at a definite position (Gly(A1) or Lys(B29)) on the molecule is described. Gly(A1)- and Lys(B29)-S-acetylthioglycoloyl-insulins and Gly(A1)-2(or 3)-acetylmercapto-3-carboxypropanoyl-insulin were deacetylated and then reacted with 7-chloro-4-sulfobenzoxadiazole, a fluorogenic reagent, to afford the corresponding fluorescence labeled insulins. The preparative separation of the fluorescence labeled insulins from the insulin derivatives that remained unreacted was carried out by anion-exchange high-performance liquid chromatography on a TSKgel DEAE-2SW column.
关键词：fluorescence labeled insulin;7-chloro-4-sulfobenzoxadiazole;anion-exchange HPLC;acetylthioglycoloyl insulin;acetylmercapto-3-carboxypropanoyl insulin