文章基本信息

标题：Preparation and Evaluation of Fatty Acid Esters of 2-(4-Hydroxyphenyl)-4, 5-diphenylimidazole as Fluorescent Substrates for Measurement of Lipase Activity
本地全文：下载
作者：Naotaka KURODA ; Mioko TAKATANI ; Kenichiro NAKASHIMA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：3
页码：220-222
DOI：10.1248/bpb.16.220
出版社：The Pharmaceutical Society of Japan
摘要：A series of four aliphatic carboxylic acid esters (acetate, caproate, laurate and palmitate) of 2-(4-hydroxyphenyl)-4, 5-diphenylimidazole (HDPI) was prepared. Usefulness of these esters as fluorescent substrates for the measurement of lipase activity was evaluated using a high performance liquid chromatographic method. The hydrolysate, HDPI, could be well separated from each substrate by a reversed-phase column and detected at 440 nm emission (cutoff-filter) with 360 nm excitation. Lipase from Candida cylindracea was used for the investigation. From their kinetic parameters, the caproate and laurate were found to be suitable for the lipase. Using the laurate, from 5 to 500 mU per assay tube of the lipase was determined.
关键词：lipase activity;fluorescent substrate;2-(4-hydroxyphenyl)-4, 5-diphenylimidazole;HPLC assay;lipase;carboxylic acid