文章基本信息

标题：Studies on Thermophile Products. VI. Activation of Mouse Peritoneal Macrophages by Bis(2-hydroxyethyl) Trisulfide
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作者：Yasuhiro KOHAMA ; Kentaro IIDA ; Keishi TANAKA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：10
页码：973-977
DOI：10.1248/bpb.16.973
出版社：The Pharmaceutical Society of Japan
摘要：The biological effects of a cytotoxic substance (BS-1), isolated from Bacillus stearothermophilus UK563 and identified as bis(2-hydroxyethyl) trisulfide, on elicited mouse peritoneal macrophages induced by a casein injection, were investigated in vitro. BALB/c mouse macrophages treated or pretreated with BS-1 (1-10 μg/ml) showed cytotoxicity against syngeneic DBA/2 mouse P815 mastocytoma. BS-1 also showed weak cytotoxicity directly against P815 in the absence of macrophages. BS-1 significantly increased the glucose consumption of macrophages without producing cytotoxicity. This trisulfide compound increased nitric oxide formation, interleukin-1 production and prostaglandin E₂ release in macrophages. It did not, however, increase the production of active oxygen species in macrophages, but it reduced cytochrome c in the presence of phagocytes. These results indicate that BS-1 activates macrophages to the cytolytic stage.
关键词：bis(2-hydroxyethyl) trisulfide;cytotoxicity;macrophage activation;glucose consumption;nitric oxide;IL-1