文章基本信息

标题：Modification of Liposomes by Addition of HCO60. II. Encapsulation of Doxorubicin into Liposomes Containing HCO60
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作者：Yasuki KATO ; Toshihito HOSOKAWA ; Yuichi OKUBO 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：10
页码：965-969
DOI：10.1248/bpb.16.965
出版社：The Pharmaceutical Society of Japan
摘要：The efficiency of encapsulation of doxorubicin (DOX) into liposomes containing HCO60 was studied. Empty egg phosphatidylcholine (PC) liposomes containing various amounts of HCO60 were passed through a polycarbonate membrane filter (0.1 μm). Closed vesicles were formed even with liposomes containing 90 wt% HCO60. DOX was encapsulated efficiently (more than 1 mg DOX/8 mg lipids) using a pH-gradient method. HCO60 accelerated the leakage of DOX from liposomes into phosphate buffer at temperatures above 25°C. HCO60 suppressed the leakage of DOX from egg PC/cholesterol (7/3, w/w) liposomes into phosphate buffer containing 50% fetal bovine serum (FBS). Studies on the interaction between liposomes and serum components using zeta potential and turbidity suggest that HCO60 prevents the liposomes from interacting with serum components.
关键词：liposome;nonionic surfactant;doxorubicin;pH gradient;encapsulation