文章基本信息

标题：Characterization of Lignoceroyl-CoA Ligase Activity in Chicken Liver Microsomes
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作者：Takayuki SHIRAISHI ; Yutaka UDA
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：10
页码：956-959
DOI：10.1248/bpb.16.956
出版社：The Pharmaceutical Society of Japan
摘要：Lignoceroyl-coenzyme A (CoA) ligase activity was detected in microsomal fractions from chicken liver in the presence of α-cyclodextrin as a solubilizing agent of lignoceric acid. Heptakis(2, 6-di-O-methyl)-β-cyclodextrin (dimethyl-β-cyclodextrin) and hexakis(2, 6-di-O-methyl)-α-cyclodextrin (dimethyl-α-cyclodextrin), among the cyclodextrins tested, were more effective than α-cyclodextrin as solubilizing agents. We have characterized lignoceroyl-CoA ligase activity in comparison with palmitoyl-CoA ligase activity. Lignoceroyl-CoA and palmitoyl-CoA ligase activities showed a similar dependency on CoA concentration. However, lignoceroyl-CoA ligase activity exhibited responses to the Mg²⁺ ion, adenosine triphosphate (ATP), ATP analogues (adenosine monophosphate (AMP) and adenosine diphosphate (ADP)), and heat treatment, which were distinctly different from the responses of palmitoyl-CoA ligase activity. These results are consistent with the idea that lignoceroyl-CoA and palmitoyl-CoA are synthesized by two different enzymes.
关键词：lignoceric acid;very-long-chain fatty acyl-CoA ligase;long-chain fatty acyl-CoA ligase;heptakis(2, 6-di-O-methyl)-β-cyclodextrin