文章基本信息

标题：Comparison of the Effect of β-Alanyl-L-histidinato Zinc and Its Zinc-Chelating Ligand on Bone Metabolism in Tissue Culture
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作者：Masayoshi YAMAGUCHI ; Seiko KISHI
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1994
卷号：17
期号：4
页码：522-526
DOI：10.1248/bpb.17.522
出版社：The Pharmaceutical Society of Japan
摘要：The present investigation was undertaken to compare the effects of β-alanyl-L-histidinato zinc (AHZ) and its zinc-chelating ligands on bone metabolism in tissue culture. Calvaria were removed from 3-week-old male rats and cultured for up to 72h in Dulbecco's modified eagle medium containing zinc sulfate, AHZ, di (N-acetyl-β-alanyl-L-histidinato) zinc (AAHZ), and di (histidino) zinc (HZ). The bone calcium content and alkaline phosphatase activity were significantly increased in the presence of AHZ or AAHZ (10^-8-10^-5 M). Those increases were seen at 10^-7 to 10^-5 M zinc sulfate and HZ. The bone deoxyribonucleic acid (DNA) content was significantly increased by AHZ or AAHZ with 10^-7 to 10^-5 M, while 10^-7 M zinc sulfate and HZ had no effect. Thus, AHZ and AAHZ had more potent effect than that of zinc sulfate and HZ. The effect of AHZ, AAHZ and HZ (10^-5 M) increasing bone alkaline phosphatase activity was abolished by the presence of 10^-4 M dipicolinate, a chelator of zinc. Moreover, the effect of these zinc compounds on bone metabolic indices was not seen in the presence of 10^-6 M cycloheximide. The present results suggest that the effect of AHZ and AAHZ on bone metabolism is more potent than that of zinc sulfate and HZ.