文章基本信息

标题：Divergent Trophic Actions of Glioma Conditioned Media on Cultured Rat Hippocampal Neurons
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作者：Shoki OKUDA ; Hiroshi SAITO ; Hiroshi KATSUKI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1994
卷号：17
期号：5
页码：735-738
DOI：10.1248/bpb.17.735
出版社：The Pharmaceutical Society of Japan
摘要：Neuron-glia interaction is considered to be important for the establishment and maintenance of neuronal shape and polarity during development. Glial cells release a number of trophic factors, many of which have not been fully characterized. In the present study, the trophic influences of conditioned media (CMs) of four glioma cell lines (T98G, Hs683, AC, C6) were tested on cultured hippocampal neurons dissociated from fetal rat brain. The survival of hippocampal neurons was enhanced by C6-CM, but inhibited by T98G-CM. The elongation of neurites was remarkably potentiated by C6-CM and T98G-CM. The other two CMs had little effect on neuronal survival and neurite elongation. These results suggest that different glioma cell lines produce and release different kinds and/or amounts of neurotrophic factors. Our experimental method may provide a suitable assay system for the identification of unknown trophic factors.
关键词：trophic factor;glioma;conditioned medium;hippocampal neuron