文章基本信息

标题：Evidence for Accumulation of Lipid Hydroperoxides during the Aging of Human Red Blood Cells in the Circulation
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作者：Ken ANDO ; Masatoshi BEPPU ; Kiyomi KIKUGAWA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1995
卷号：18
期号：5
页码：659-663
DOI：10.1248/bpb.18.659
出版社：The Pharmaceutical Society of Japan
摘要：Young and old human red blood cells (RBC) were separated from freshly collected human blood by Percoll density gradient centrifugation. The amounts of lipid peroxidation products in the lipid fractions of these RBC were determined by high performance liquid chromatography-chemiluminescence, thiobarbituric acid and LPO-586 methods. The levels of phosphatidylcholine and phosphatidylethanolamine hydroperoxides in old RBC were higher than in young RBC. The products reflecting the advanced stage of lipid peroxidation, malonaldehyde (free and bound forms), 4-hydroxyalkenals (free and bound forms) and other aldehydes, were also higher in old RBC than in young RBC. The levels of these lipid peroxidation products increased when whole RBC were mildly oxidized with ferric ion. These results indicate that oxidative damage of RBC in the circulation takes place during the aging process, and lipid hydroperoxides and other lipid peroxidation products accumulate in old RBC.
关键词：old red blood cell;phosphatidylcholine hydroperoxide;phosphatidylethanolamine hydroperoxide;thiobarbituric acid reactive substance;malonaldehyde;4-hydroxyalkenal