文章基本信息

标题：Study of the de Novo Synthesis of Cholesterol in the Rat Liver : A Newly Developed Radiotracer Technique, "TLC-Autoradioluminography"
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作者：Mitsunobu OKUYAMA ; Masanobu TSUNOGAI ; Noriko WATANABE 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1995
卷号：18
期号：11
页码：1467-1471
DOI：10.1248/bpb.18.1467
出版社：The Pharmaceutical Society of Japan
摘要：A possible evaluation technique was devised for examing timely cholesterol synthetic activity in the rat liver. The principle of the technique is to measure the incorporation rate of ¹⁴C atoms into a few target metabolites excreted in bile with time after an intraportal injection of [1, 2-¹⁴C] acetate to the conscious rats. The measuring technique, TLC-autoradioluminography (TLC-ARLG), was devised for much more accurate, quantitative, simple and quick ultramicroanalysis of radioactive metabolites than ever before. Practically, a few μI of the bile samples were analyzed by TLC before recording of a 2-dimensional radioactive image on the TLC plate. The incorporation ratio of ¹⁴C into cholesterol to succinic acid (C/S ratio) varied such that the value found in a 24-h fasting group was less than one-tenth of that in a sufficiently fed control group. For use as an antihyperglycemic drug, the bile C/S ratio in the nicotinic-acid-dosed group was approximately one-fifth of that in the control group.
关键词：cholesterol de novo synthesis;quantitative TLC-autoradioluminography;bile metabolite