文章基本信息

标题：Application of [125I] (S)-5-Iodonicotine, a New Radioiodinated Ligand, in the Assay of Nicotinic Acetylcholine Receptor Binding in the Brain
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作者：Hideo SAJI ; Akira WATANABE ; Yasushi KIYONO 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1995
卷号：18
期号：11
页码：1463-1466
DOI：10.1248/bpb.18.1463
出版社：The Pharmaceutical Society of Japan
摘要：[¹²⁵I] (S)-5-Iodonicotine was prepared and its application in the assay of nicotinic acetylcholine receptor binding in the brain was studied. [¹²⁵I] (S)-5-Iodonicotine bound to the rat cortical membrane with high affinity (K_d, 15.0nM). Various nicotinic cholinergic compounds showed competition with [¹²⁵I] (S)-5-iodonicotine for the binding sites in the rat cortical membrane, and the specificity of its binding was correlated well with that of [³H] cytisine (r=0.98). These findings suggest that [¹²⁵I] (S)-5-iodonicotine binds to the same sites as [³H] cytisine and indicate that [¹²⁵I] (S)-5-iodonicotine can be applied as a brain nicotine receptor binding assay.
关键词：radioiodinated (S)-nicotine analog;radioreceptor assay;brain nicotine receptor;iodine-125;tritiated cytisine