文章基本信息

标题：Apolipoprotein B Inhibits the Hemolytic Activity of Asp-Hemolysin from Aspergillus fumigatus
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作者：Yuji FUKUCHI ; Takeshi KUMAGAI ; Keiichi EBINA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：4
页码：547-550
DOI：10.1248/bpb.19.547
出版社：The Pharmaceutical Society of Japan
摘要：We investigated the effect of human blood plasma lipoproteins on the hemolytic activity of Asp-hemolysin. Apolipoprotein B (apoB)-containing lipoproteins, such as low density lipoprotein (LDL) and very low density lipoprotein (VLDL), inhibit the activity of this hemolytic toxin. When Asp-hemolysin (15 μg) was incubated with 100 μg LDL (as protein), the hemolytic activity was inhibited by 90%. When 20 μg apoB was added, the hemolytic activity was almost completely inhibited. Furthermore, similar inhibition was obtained in the filtrates which were separated from the incubation mixture of Asp-hemolysin with LDL or apoB following ultrafiltration through a membrane with a molecular cutoff=100000. The current findings suggest that the inhibition by LDL is due to apoB binding to Asp-hemolysin.
关键词：Asp-hemolysin;hemolytic activity;low density lipoprotein (LDL);very low density lipoprotein (VLDL);apolipoprotein B (apoB)