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标题：Separation and Characterization of Monoglucuronides of Vitamin D3 and 25-Hydroxyvitamin D3 in Rat Bile by High-Performance Liquid Chromatography
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作者：Kazutake SHIMADA ; Ito NAKATANI ; Kyoko SAITO 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：4
页码：491-494
DOI：10.1248/bpb.19.491
出版社：The Pharmaceutical Society of Japan
摘要：The separation and characterization of vitamin D₃- and 25-hydroxyvitamin D₃-monoglucuronides, biliary metabolites obtained from rats dosed with D₃ and 25-hydroxyvitamin D₃ per os, respectively, were carried out by HPLC. The glucuronide fractions were obtained from bile specimens by the combined use of a Bond Elut C18 cartridge, for solid phase extraction, and a lipophilic gel (piperidinohydroxypropyl Sephadex LH-20), for ion-exchange chromatography. Each glucuronide was identified by comparison with an authentic sample in three ways : its chromatographic behavior, that of its fluorescent labeled derivative using 4-[4-(6-methoxy-2-benzoxazolyl)phenyl]-1, 2, 4-triazoline-3, 5-dione and data obtained following enzymatic hydrolysis using β-glucuronidase.
关键词：monoglucuronide;vitamin D3;25-hydroxyvitamin D3;rat;biliary metabolite;identification