文章基本信息

标题：Assay for Tryptophan Hydroxylase Activity in Rat Brain by High-Performance Liquid Chromatography with Fluorescence Detection
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作者：Ryuji IIZUKA ; Junichi ISHIDA ; Takashi YOSHITAKE 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：5
页码：762-764
DOI：10.1248/bpb.19.762
出版社：The Pharmaceutical Society of Japan
摘要：A highly sensitive and selective liquid chromatographic (HPLC) method with post-column fluorescence detection has been developed for the determination of tryptophan 5-monooxygenase activity in rat brain tissue homogenate. 5-Hydroxytryptophan, formed enzymatically from tryptophan (incubation time, 20 min), is extracted with perchloric acid and determined by HPLC. Detection is performed fluorometrically after post-column derivatization by a reaction with benzylamine in the presence of potassium hexacyanoferrate (III). The lower limit of detection for 5-hydroxytryptophan formed enzymatically is 100 fmol at a signal-to-noise ratio of three.
关键词：tryptophan 5-monooxygenase;5-hydroxyindole;HPLC;fluorescence;rat brain