文章基本信息

标题：A New Method for Permeabilization of the Plasma Membrane of Cultured Mammalian Cells. V. A Standard Procedure Recommended for Permeabilization by Vortex-Stirring with High Molecular Weight Polyacrylic Acid
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作者：Naoaki SHIMIZU ; Yutaka KAWAZOE
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：10
页码：1279-1284
DOI：10.1248/bpb.19.1279
出版社：The Pharmaceutical Society of Japan
摘要：As we previously reported, permeabilization of the plasma membrane was effectively achieved by vortex-stirring the mammalian cells in the presence of high molecular weight polyacrylic acid. Based on our present findings, as well as those of our previous studies, optimal conditions for permeabilization were considered in terms of the structure and dosage of a polymer, the constituent of the medium, the conditions of cells, vortex-stirring and post-vortex-stirring, etc. A standard procedure tentatively recommended is described.
关键词：permeabilization;mammalian cell;polyacrylic acid;plasma membrane;vortex-stirring