文章基本信息

标题：Growth Phenotypes of Escherichia coli Carrying a Mutation of Acidic Phospholipid Synthesis
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作者：Nobuyoshi AKIMITSU ; Tohru MIZUSHIMA ; Emi SUZUKI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：10
页码：1275-1278
DOI：10.1248/bpb.19.1275
出版社：The Pharmaceutical Society of Japan
摘要：We identified novel phenotypes of a pgsA3 mutant lacking the potential to synthesize phosphatidylglycerolphosphate, a precursor of phosphatidylglycerol. The first phenotype is limited cell growth at a high temperature, under the condition of low salt. The phenotype was co-transduced with a phenotype lacking the potential to synthesize phosphatidylglycerol in a P1 transduction experiment, and was restored by transformation with a plasmid containing a wild type pgsA gene. The second phenotype of the pgsA3 mutant was resistant to growth in the presence of a low concentration of kanamycin (4 μg/ml). P1 transduction and transformation with the plasmid containing the wild-type pgsA gene revealed that the pgsA3 mutation was also responsible for the second phenotype.
关键词：temperature-sensitive growth;kanamycin;phosphatidylglycerol;Escherichia coli