文章基本信息

标题：Failure of Smooth Muscle Cells Treated with Collagenase to Respond to Bordetella Heat-Labile Toxin
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作者：Masaaki NAGAI ; Mineo WATANABE ; Masahiko ENDOH 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：11
页码：1508-1510
DOI：10.1248/bpb.19.1508
出版社：The Pharmaceutical Society of Japan
摘要：HLT, Bordetella heat-labile toxin, caused a release of radioactivity from cultured smooth muscle cells (SMC) previously labeled with [¹⁴C]arachidonic acid, and from membrane preparations from the labeled SMC. HLT, however, failed to induced release from primary SMC prepared by treatment with an enzyme solution containing collagenase and elastase. When cultured SMC were treated with the enzyme solution, their susceptibility to HLT was reduced more significantly than that of untreated cells. SMC also lost their susceptibility, after treatment with collagenase alone, but not with elastase, in a dose-dependent manner. These data suggest that collagen on the SMC surface might play an important role in HLT activity.
关键词：Bordetella;heat-labile toxin;smooth muscle cell;collagen;collagenase