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标题：Participation of Leukotriene D4 and Tumor Necrosis Factor on Lipopolysaccharide-Induced Airway Hyperresponsiveness in Guinea Pigs
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作者：Takashi UNO ; Hiroyuki TANAKA ; Noriko NAKAI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1997
卷号：20
期号：4
页码：332-337
DOI：10.1248/bpb.20.332
出版社：The Pharmaceutical Society of Japan
摘要：In guinea pigs, a marked increase in airway responsiveness to acetylcholine (Ach) was observed at 2h after lipopolysaccharide (LPS) inhalation. To examine the mediators responsible for the airway hyperresponsiveness, the changes of peptide-leukotrienes (LTs), tumor necrosis factor (TNF), interleukin-1 (IL-1), histamine and 5-hydroxytryptamine (5-HT) levels in bronchoalveolar lavage fluid (BALF) were measured. Airway responsiveness to Ach reached a peak 2h after LPS inhalation. The influx of neutrophil into BALF increased gradually and reached a peak 24h after LPS inhalation. After the inhalation of LPS, LTD₄ and TNF contents in BALF increased within the first 2h after LPS inhalation. However, other mediators were not detected or increased 6h after LPS inhalation. Aeroinhalation of LTD₄ and murine recombinant TNF-α caused airway hyperresponsiveness in guinea pigs. In addition, a LTD₄ antagonist, BAYx7195, and an inhibitor of TNF, pentoxifylline, inhibited the LPS-induced airway hyperresponsiveness. These results suggest that LTs and/or TNF play an important role in the onset of airway hyperresponsiveness in guinea pigs.
关键词：lipopolysaccharide;airway hyperresponsiveness;leukotriene;tumor necrosis factor