文章基本信息

标题：Improved Separation and Determination of Phospholipids in Animal Tissues Employing Solid Phase Extraction
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作者：Emi SUZUKI ; Akimitsu SANO ; Takeo KURIKI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1997
卷号：20
期号：4
页码：299-303
DOI：10.1248/bpb.20.299
出版社：The Pharmaceutical Society of Japan
摘要：Separation of five glycerophospholipids having different polar groups, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylglycerol (PG) and cardiolipin (CL), was investigated by means of solid-phase extraction (SPE) cartridges. First, the phospholipids were retained in an aminopropylbonded phase (NH₂) cartridge and subsequently eluted as neutral (PC and PE) and acidic (PS, PG and CL) glycerophospholipid fractions. Secondly, a combination of silica gel (SI) cartridge and NH₂ cartridge was employed to separate five glycerophospholipids. The polarity of the eluent was responsible for neutral glycerophospholipid separation. Concerning acidic glycerophospholipids, the separation of PG and CL from PS depended mainly on the pH of the eluents, and the separation of PG and CL was affected by the solvent, depending on eluent polarities. Favorable recovery (not less than 95%, for five authentic phospholipids, 10-100 μg each) and repeatability (σ=2.3 for 10 μg ranges) were attained by the present method. This method of separation was applicable to the analysis of phospholipids in biological samples.
关键词：solid phase extraction;neutral phospholipid;acidic phospholipid;capillary GC analysis;fatty acid composition