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标题：Gastric Mucin Secretion from Cultured Rat Epithelial Cells
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作者：Satoru TANI ; Masaki OKUDA ; Rie MORISHIGE 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1997
卷号：20
期号：5
页码：482-485
DOI：10.1248/bpb.20.482
出版社：The Pharmaceutical Society of Japan
摘要：In order to establish the measurement of gastric mucin secreted from cultured mucous cells, rat gastric mucin was purified from secreted mucus with Sepharose CL-4B column chromatography. Gastric mucin was measured by dot blot analysis using an enzyme-linked lectin (soybean agglutiin) assay in a good concentration-dependent manner. Surface epithelical cells were dispersed by limited digestion of a rat everted stomach and collected by density gradient centrifugation with Percoll. These cells were inoculated onto gelled collagen dishes, then cultured in a medium supplemented with 10% fetal calf serum under a 5% CO₂ atomosphere in air. Changing the medium after a 2-d culture, the cells were cultured for another 3 d. During the culture, the numbers of cells each day were almost, equal, but mucin contents in the cells increased, and then dropped at day 5 after inoculation. At that time, the edge of the cell layer peelede off and the cells adhered to each other. Using 2-d cultured cells, the effects of some secretagogues on mucin secretion were investigated. Carbachol, secretin, CCK-8 and prostaglandin E₂ (PGE₂) strongly stimulated mucin secretion, and gastrin I weakly did. However, histamine offered no stimulation.
关键词：gastric mucin;epithelial cell;secretion;mucus;carbachol;secretin