文章基本信息

标题：Dual Regulation of Alkaline Phosphatase Activity by Calcitonin in Porcine Kidney Cells
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作者：Ken-ichi MIYAMOTO ; Takashi HORITA ; Yoshihiro WAKI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1997
卷号：20
期号：12
页码：1300-1302
DOI：10.1248/bpb.20.1300
出版社：The Pharmaceutical Society of Japan
摘要：Intracellular signal transduction for regulation of alkaline phosphatase (ALP) activity in renal epithelial cells treated with calcitonin is not yet completely understood, although it is known that calcitonin receptors couple to cyclic AMP-dependent protein kinase (PKA) and calcium/phospholipid-dependent protein kinase (PKC). Salmon calcitonin increased the cyclic AMP content in LLC-PK₁ porcine kidney cells in a concentration-dependent manner. When the confluent cells were incubated for 47 h after a 1 h-pulse exposure or continuously exposed to calcitonin and forskolin for 48 h, ALP activity in the cells was increased by calcitonin about 2-fold compared with the basal acitivity at the maximum level but was not dependent on the exposure time; it was markedly increased by forskolin in parallel with the exposure time. The increase in activity produced by calcitonin was abolished by a PKA inhibitor H-89, and, in contrast, potentiated by a PKC inhibitor, NA-382 to near the forskolin-induced level. These results indicate that calcitonin exerts a dual-regulation of ALP activity in LLC-PK₁ cells, positively through the PKA pathway and negatively through PKC.
关键词：calcitonin;alkaline phosphatase;protein kinase;renal epithelial cell;LLC-PK1 cell