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  • 标题:Real Time Measurement of Nitric Oxide Released from Cultured Endothelial Cells
  • 本地全文:下载
  • 作者:Hajimu KURUMATANI ; Kazuya KIKUCHI ; Tetsuo NAGANO
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:1998
  • 卷号:21
  • 期号:12
  • 页码:1286-1289
  • DOI:10.1248/bpb.21.1286
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:Direct detection of nitric oxide (NO) is essential for understanding the precise mechanism of its production from endothelial cells. Previously, we developed an NO detection system based on the chemiluminescence reaction between NO and luminol-H2O2. Here, we have applied this system to cultured endothelial cells for the direct and on-time measurement of NO. The perfusate from cultured endothelial cells was continuously mixed with luminol-H2O2. NG-monomethyl-L-arginine (L-NMMA) (10-4M) decreased the chemiluminescence signal of NO, suggesting the existence of basal NO release. Bradykinin (10-8-10-6M) increased the NO signal (10-6M;5.1±0.4 fmol/min, corresponding to 1.7 pM in the perfusate), and this was inhibited by 10-4M L-NMMA (1.8±0.3 fmol/min). These results corresponded to the changes in cGMP levels in RFL-6 cells, which provide an No bioassay system. We conclude that the luminol-H2O2 system is useful for the direct and continuous measurement of NO from coltured endothelial cells.
  • 关键词:nitric oxide;endothelium;bradykinin;chemiluminescence;luminol
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