文章基本信息

标题：β-Thujaplicin Zinc Chelate Induces Apoptosis in Mouse High Metastatic Melanoma B16BL6 Cells
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作者：Daisei MIYAMOTO ; Naoko ENDO ; Naoto OKU 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1998
卷号：21
期号：12
页码：1258-1262
DOI：10.1248/bpb.21.1258
出版社：The Pharmaceutical Society of Japan
摘要：The cytotoxic effects of β-thujaplicin and five kinds of metal chelates were examined on mouse melanoma B16BL6 cells by cell viability and lactate dehydrogenase (LDH) release assay. β-Thujaplicin-zinc chalate and β-thujaplicin-copper chelate had higher cytotoxic effects than β-thujaplicin, and the 50% effective does (ED₅₀) of these metal chelates were 12.5 and 25 μM, respectively. In addition, the zinc chelate induced DNA ladder formation in B16BL6 cells, as shown by the DNA fragmentation assay, suggesting that cell death induced by the zinc chelate is apoptosis. The zinc chelate also had a cytotoxic effect and induced DNA fragmentation on other tumor cell lines : HeLa, Meth A, and B16F1 cells, but not on normal human diploid fibroblasts FS-4. These results suggest that β-thujaplicin-zinc chelate induces apoptotic cell death in various tumor cell lines and is a potent antitumor agent for tumor cells including malignant melanomas.
关键词：β-thujaplicin;β-thujaplicin-zinc chelate;DNA ladder formation;apoptosis;B16BL6 melanoma