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标题：Biosynthesis of Thiamin under Anaerobic Conditions in Saccharomyces cerevisiae
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作者：Keiko TANAKA ; Keiko TAZUYA ; Kazuko YAMADA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2000
卷号：23
期号：1
页码：108-111
DOI：10.1248/bpb.23.108
出版社：The Pharmaceutical Society of Japan
摘要：We studied the biosynthetic route of thiamin in Saccharomyces cerevisiae to see whether the route differed under aerobic and anaerobic conditions.Histidine and pyridoxine are the precursors of the pyrimidine moiety of thiamin under aerobic conditions. Formate is incorporated into the pyrimidine via histidine. The incorporation of [¹³C]formate and [5'-²H₂]pyridoxine into the pyrimidine was wxaminied under anaerobic donritions. The labels from [¹³C]formate and [5'-²H₂]pyridoxine were not incorporated into the pyrimidine under anaerobic conditions, indicating that the biosynthetic pathway of the pyrimidine differed from that under aerobic conditions.On the other hand, [¹⁵N]glycine was incorporated into the thiazole under both anaerobic and aerobic conditions. The biosynthetic pathway of the thiazole was therefore unaltered by the O₂ concentration.
关键词：thiamin;biosynthesis;anaerobic conditions;Saccharomyces cerevisiae