文章基本信息

标题：Molecular Cloning and Characterization of a cDNA for Glycyrrhiza glabra Cycloartenol Synthase
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作者：Hiroaki HAYASHI ; Noboru HIRAOKA ; Yasumasa IKESHIRO 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2000
卷号：23
期号：2
页码：231-234
DOI：10.1248/bpb.23.231
出版社：The Pharmaceutical Society of Japan
摘要：A cDNA clone (GgCAS1) encoding cycloartenol synthase (CAS) has been isolated from Glycyrrhiza glabra(licorice) by cross-hybridization with that of Pisum sativum CAS as a probe. The deduced amino acid sequence of GgCAS1 exhibits 89%, 83% and 81% identity to those of Pisum sativum, Panax ginseng and Arabidopsis thaliana CASs, respectively. CAS activity has been detected in the homogenate of the yeast transformed with the expression vector containing the open reading frame of GgCAS1. Southerbn blot analysis suggested that at least two CAS genes exist in the licorice genome. In Northern blot analysis, the strong signal for CAS mRNA is detected in the cultured licorice cells of all growth phases, but no significant increase of GAS mRNA expression was observed in the cells treated with the 3-hydroxy-3-methylglutaryl-CoA reductase inhibitor, pravastatin.
关键词：Glycyrrhiza glabra;cDNA cloning;cycloartenol synthase;phytosterol