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标题：Inhibitory Effects of Green Tea and Grape Juice on the Phenol Sulfotransferase Activity of Mouse Intestines and Human Colon Carcinoma Cell Line, Caco-2
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作者：Hiro-omi TAMURA ; Michio MATSUI
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2000
卷号：23
期号：6
页码：695-699
DOI：10.1248/bpb.23.695
出版社：The Pharmaceutical Society of Japan
摘要：Tea and fruit juices are beverages consumed daily all over the world. The present study reports the inhibitory effects of these beverages on the activity of mammalian intestinal phenol sulfotransferases (P-STs).Green tea strongly inhibited the E. coli-expressed mouse intestinal P-ST activity in vitro. (-)-Epigallocatechin gallate (EGCG) was found to be the most potent inhibitor among the catechins tested (IC₅₀=0.93 μM). (-)EGCG also inhibited the P-ST activity of the human colon carcinoma cell line, Caco-2. Kinetic analysis showed that the inhibition was competitive. Among fruit juices examined (apple, grape, grapefruit and orange), grape juice exhibited the most potent inhibitory action on the P-ST activity of mouse intestines and human colon carcinoma cells. The inhibitory activity of grape juice was located mainly in the skin and seels. Flavonols, such as quercetin and kaempferol, inhibited the P-ST activity at low concentrations. These observations suggest the possible inhibition of P-St activity in human intestines by green tea or grape juice.
关键词：Caco-2;catechin;green tea;flavonol;inhibition;sulfotransferase