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标题：Inhibitory Effect of Gnetin C, a Resveratrol Dimer from Melinjo (Gnetum gnemon), on Tyrosinase Activity and Melanin Biosynthesis
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作者：Miyako Yanagihara ; Maiko Yoshimatsu ; Akinori Inoue 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2012
卷号：35
期号：6
页码：993-996
DOI：10.1248/bpb.35.993
出版社：The Pharmaceutical Society of Japan
摘要：Tyrosinase is the key enzyme involved in melanogenesis. The aim of this study was to investigate the in vitro inhibitory effects of gnetin C, a resveratrol dimer isolated from melinjo ( Gnetum gnemon ) seeds, on tyrosinase activity and melanin biosynthesis in murine B16 cells. The inhibitory activities of gnetin C and resveratrol were shown to be almost equal against tyrosinase and melanin biosynthesis in the cells. The IC₅₀ values of gnetin C activity against tyrosinase and melanin biosynthesis were 7.0 and 7.6 µ M , respectively, whereas resveratrol demonstrated IC₅₀ values of 7.2 and 7.3 µ M , respectively. These results indicated that gnetin C inhibited melanogenesis, in a manner similar to that of resveratrol, by inhibiting tyrosinase and may therefore function as a new skin-whitening agent. However, the direct effects of gnetin C and resveratrol on murine tyrosinase activities were not equal. The IC₅₀ value of resveratrol was 10.1 µ M , while gnetin C only exhibited a 25.2% enzyme inhibition at 16 µ M . The IC₂₅ values for gnetin C and resveratrol were 15.5 and 4.0 µ M , respectively. Therefore, it is suggested that the effects of gnetin C may be due to mechanisms other than the direct inhibition of tyrosinase activity.
关键词：gnetin C;tyrosinase;melanin;melinjo;resveratrol dimer