文章基本信息

标题：Dysfunction of Neurotransmitter Modulation System on Adrenergic Nerves of Caudal Artery in Type 2 Diabetic Goto–Kakizaki Rats
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作者：Reiko Ishii-Nozawa ; Mitsuo Mita ; Masaru Shoji 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2012
卷号：35
期号：7
页码：1091-1095
DOI：10.1248/bpb.b12-00052
出版社：The Pharmaceutical Society of Japan
摘要：The Goto–Kakizaki (GK) rat is a non-obese and spontaneous model of mild Type 2 diabetes mellitus. In the present study, we compared the regulatory mechanisms of endogenous norepinephrine (NE) release from sympathetic nerves of caudal arteries of 12-week-old GK rats and age-matched normal Wistar rats. Electrical stimulation (ES) evoked significant NE release from caudal arteries of Wistar and GK rats. The amounts of NE released by ES were almost equal in Wistar and GK rats, although the NE content in caudal artery of GK rats was significantly lower than that of Wistar rats. We examined the effects of an α₂-adrenoceptor agonist, clonidine (CLO), and an α₂-adrenoceptor antagonist, yohimbine (YOH), on the release of endogenous NE evoked by ES. CLO significantly reduced NE release from caudal arteries of Wistar but not GK rats. On the other hand, YOH significantly increased NE release from both rats. Furthermore, we examined the effects of an A₁-adenosine receptor agonist, 2-chloroadenosine (2CA), and an A₁-adenosine receptor antagonist, 8-sulfophenyltheophylline (8SPT), on the release of endogenous NE evoked by ES. 2CA significantly reduced NE release from caudal arteries of Wistar but not GK rats. On the other hand, 8SPT did not affect NE release from both rats. These results suggest that the dysfunction of negative feedback regulation of NE release via presynaptic receptors on sympathetic nerves in GK rats may be involved in the autonomic nervous system dysfunction associated with diabetic autonomic neuropathy.
关键词：adrenergic nerve;diabetes mellitus;autonomic neuropathy;norepinephrine release;presynaptic receptor;rat caudal artery