文章基本信息

标题：Intracellular Internalization Mechanism of Protein Transfection Reagents
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作者：Makoto Oba ; Masakazu Tanaka
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2012
卷号：35
期号：7
页码：1064-1068
DOI：10.1248/bpb.b12-00001
出版社：The Pharmaceutical Society of Japan
摘要：A protein transfection reagent is a powerful tool for elucidating a protein function in a cell, and plays an important role in the fields of cell biology and drug discovery. Many researchers have developed protein delivery systems and several systems are commercially available. In this study, we focus on the biological functions of three commercially available protein transfection reagents, Pro-DeliverIN, Xfect, and TurboFect, especially in their internalization routes by HeLa cells. A cellular uptake study using specific endocytosis inhibitors and confocal laser scanning microscope observation revealed that each reagent was internalized into HeLa cells by different mechanism. It is our hope that the results presented here will help in the choice and use of protein transfection reagents for experiments.
关键词：protein transfection reagent;intracellular internalization;endocytosis;protein delivery system