文章基本信息

标题：A Rapid Method Using QUALIBAX^TM System for Detecting Salmonella in Oil Meal
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作者：Hideki Kitazawa ; Yousuke Yukinawa ; Hiroshi Aoki 等
期刊名称：日本食品微生物学会雑誌
印刷版ISSN：1340-8267
电子版ISSN：1882-5982
出版年度：2017
卷号：34
期号：1
页码：13-20
DOI：10.5803/jsfm.34.13
出版社：Japanese Society of Food Microbiology
摘要：Oil meal, which is used as a feed, is a byproduct of food oil production, and knowledge on Salmonella contamination in oil meal is essential in the hygiene management of domestic animals. Moreover, the rapid on-site detection of Salmonella is necessary. Thus, in the present study, we aimed to investigate a rapid detection method for Salmonella present in oil meal. When selecting appropriate media, the number of bacterial cells was significantly higher ( p <0.001) in MP than in other media, such as buffered peptone water, after 7-hr incubation. Prior to DNA analysis using the QUALIBAX™ system, QuickGene-Mini80 filtration of samples contributed to improved sensitivity of the system. Thus, the combined use of MP medium, QuickGene-Mini80 purification, and the QUALIBAX™ DNA analysis system allowed for the rapid detection of Salmonella in oil meal.
关键词：oil meal ; QuickGene ; QUALIBAX^TM system ; Salmonella