首页    期刊浏览 2024年12月02日 星期一
登录注册

文章基本信息

  • 标题:CHARACTERIZATION OF PATHOGENIC E. COLI ISOLATED IN SHEEP AT SLAUGHTERHOUSE
  • 作者:R. Mazzette ; G. Busia ; A. Mureddu
  • 期刊名称:Italian Journal of Food Safety
  • 电子版ISSN:2239-7132
  • 出版年度:2012
  • 卷号:1
  • 期号:4
  • 页码:71-75
  • DOI:10.4081/ijfs.2012.4.71
  • 语种:English
  • 出版社:PAGEPress Publications
  • 摘要:Fleeces, carcass surface, mucosal gut and faeces samples, collected from 95 slaughtered sheep and lambs from three abattoirs, were examined. The aim of this study was: 1) to evaluate the prevalence of the Verocytotoxic E. coli (VTEC); 2) to obtain virulence profile (stx1, stx2, hlyA and eae) by multiplex PCR; 3) to define the ovine-specific serogroup pathogenic power for the humans. An overall prevalence of 11.1% (adults 14%, lambs 7.8%) was found by direct PCR test. The VTEC occurrence was 18.9% in fleeces, 14.7% on carcasses and 10.5% in mucosal gut. According to the multiplex PCR the following results were obtained: 21% of the isolates belonged to VTEC pathogroup, within 92% were EHEC; 37.9% were identified as EPEC pathogroup. Forty one % of the strains were negative for all the genes. None of the isolates belonged to O157 and 0146 serogroups, while the 57% resulted O91.
  • 关键词:E. coli; VTEC; PCR; sheep; serotype
Loading...
联系我们|关于我们|网站声明
国家哲学社会科学文献中心版权所有