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标题：The elaborate route for UDP-arabinose delivery into the Golgi of plants
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作者：Carsten Rautengarten ; Devon Birdseye ; Sivakumar Pattathil 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：16
页码：4261-4266
DOI：10.1073/pnas.1701894114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In plants, L-arabinose (Ara) is a key component of cell wall polymers, glycoproteins, as well as flavonoids, and signaling peptides. Whereas the majority of Ara found in plant glycans occurs as a furanose ring (Ara f ), the activated precursor has a pyranose ring configuration (UDP-Ara p ). The biosynthesis of UDP-Ara p mainly occurs via the epimerization of UDP-xylose (UDP-Xyl) in the Golgi lumen. Given that the predominant Ara form found in plants is Ara f , UDP-Ara p must exit the Golgi to be interconverted into UDP-Ara f by UDP-Ara mutases that are located outside on the cytosolic surface of the Golgi. Subsequently, UDP-Ara f must be transported back into the lumen. This step is vital because glycosyltransferases, the enzymes mediating the glycosylation reactions, are located within the Golgi lumen, and UDP-Ara p , synthesized within the Golgi, is not their preferred substrate. Thus, the transport of UDP-Ara f into the Golgi is a prerequisite. Although this step is critical for cell wall biosynthesis and the glycosylation of proteins and signaling peptides, the identification of these transporters has remained elusive. In this study, we present data demonstrating the identification and characterization of a family of Golgi-localized UDP-Ara f transporters in Arabidopsis . The application of a proteoliposome-based transport assay revealed that four members of the nucleotide sugar transporter (NST) family can efficiently transport UDP-Ara f in vitro. Subsequent analysis of mutant lines affected in the function of these NSTs confirmed their role as UDP-Ara f transporters in vivo.
关键词：Golgi apparatus ; nucleotide sugars ; membrane transport ; arabinose