文章基本信息

标题：Cytidine triphosphate:phosphatidic acid cytidyltransferase in Escherichia coli
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作者：James R. Carter
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1968
卷号：9
期号：6
页码：748-754
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：An enzyme has been found in particulate fractions of Escherichia coli that catalyzes the incorporation of cytidine triphosphate (CTP) into lipid in the presence of exogenous phosphatidic acid and Mg⁺⁺. The product has been identified enzymatically and by chromatography as cytidine diphosphate diglyceride. The reaction is optimal at a pH of 6.5 and Mg⁺⁺ concentration of 5-10 m m . The apparent K_m for CTP is 7 × 10^-4 m and for phosphatidic acid, 2 × 10^-3 m . The reaction rate falls off rapidly with time and ceases entirely after 1 hr as the result of inactivation of the system by Mg⁺⁺.
关键词：CDP diglyceride ; phospholipid biosynthesis ; physical state of reaction mixture