文章基本信息

标题：1,2-3H-Cholesterol as a tracer in studies of human cholesterol metabolism
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作者：Peter D. S. Wood ; Daphne Myers ; Yuen-Ling Lee 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1967
卷号：8
期号：4
页码：406-408
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：1,2-³H-Cholesterol was fed to a subject together with 4-¹⁴C-cholesterol at a known ³H/¹⁴C ratio. The ratio was satisfactorily preserved in cholesterol recovered from plasma, red cells, and bile, and in bile acids of bile. Isotopic fractionation was seen during thin-layer chromatographic isolation of cholesterol. In work with 1,2-³H-cholesterol or its metabolites care should be taken to recover chromatographic bands in their entirety.
关键词：cholesterol ; 1,2-³H-cholesterol ; 4-¹⁴C-cholesterol ; thin-layer chromatography ; isotope effect ; plasma ; red blood cells ; bile acids ; man