文章基本信息

标题：Separation of neutral glycosphingolipids and sulfatides by thin-layer chromatography
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作者：Vladimir P. Skipski ; Anson F. Smolowe ; Marion Barclay 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1967
卷号：8
期号：4
页码：295-299
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Two one-dimensional systems for separation of glycolipids from total lipid extracts of tissues by thin-layer chromatography are described. System I used, as adsorbent, an alkaline mixture of silica gel without CaSO₄ binder (75%) and magnesium silicate (25%), and the lipids were ``developed'' with three successive solvent mixtures. The separated compounds (from the fastest to the slowest moving) were: ceramide, ceramide monohexosides, sulfatides, ceramide dihexosides, psychosine, ceramide trihexosides, and ceramide N -acetylhexosamine trihexosides. In system II a two-step development was used on an adsorbent consisting of silica gel without CaSO₄ binder (80%) and magnesium silicate (20%). The separated compounds were: ceramides, ceramide monohexosides, and ceramide dihexosides. Psychosine and sulfatides as well as ceramide trihexosides and ceramide N -acetylhexosamine trihexosides were not separated. In both systems all neutral lipids moved to the very top of the chromatogram and phospholipids stayed at the origin. Application of systems I and II for separation of glycolipids was demonstrated on total lipid extracts from animal tissues.
关键词：glycolipids ; neutral glycolipids ; glycosphingolipids ; aminoglycolipids ; separation ; ceramide monohexosides ; ceramide dihexosides ; ceramide trihexosides ; psychosine ; sulfatides ; ceramides ; cerebrosides ; ceramide N -acetylhexosamine trihexosides ; magnesium silicate ; thin-layer chromatography