文章基本信息

标题：Pyridine nucleotide synthesis by rat adipose tissue in vitro
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作者：Richard W. Hanson ; Zigmund Z. Ziporin
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1967
卷号：8
期号：1
页码：30-37
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The synthesis of NAD and NADP by rat adipose tissue was measured in vitro. Nicotinamide-7-¹⁴C and NaH₂³²PO₄ were incorporated together into NAD with a ³²P/¹⁴C ratio of 1.82 and nicotinic-7-¹⁴C acid and NaH₂³²PO₄ with a ratio of 1.94. Nicotinic acid stimulated, by 90%, lipogenesis from glucose-U-¹⁴C by rat adipose tissue in vitro. Glucose plus insulin and refeeding for 48 hr after a 48 hr fast markedly increased the incorporation of nicotinic-7-¹⁴C into NAD in rat epididymal fat pads in vitro, but neither fructose, l -glutamine, nor insulin alone increased the synthesis of NAD in this tissue. Glucose-1-¹⁴C, ribose-1-¹⁴C, and to a greater extent glucose-6-¹⁴C are incorporated into the NAD of rat adipose tissue. Fasting followed by refeeding sharply increased the radioactivity of NAD-¹⁴C formed from glucose-1-¹⁴C and glucose-6-¹⁴C but not from ribose-1-¹⁴C. Increasing the ribose concentration from 2 m m to 10 m m increased its incorporation into adipose tissue NAD twofold. The nicotinic-7-¹⁴C acid incorporation into NAD increased over the 1st hr of incubation and remained constant for the next 3 hr. The concentration of NAD in the fat pads showed a similar response to the time of incubation. NADP concentrations increased over the entire 4 hr incubation period as did the incorporation of nicotinic-7-¹⁴C acid into NADP. The results of this study suggest that NAD is synthesized de novo by rat adipose tissue in vitro and that this synthesis is increased by factors which stimulate lipogenesis.
关键词：adipose tissue ; NAD ; NADP ; biosynthesis ; incorporation ; nicotinamide ; ³²P_i ; nicotinic acid ; effect ; lipogenesis ; coenzyme synthesis ; fasting-refeeding ; rat