文章基本信息

标题：Release of carbohydrates from sphingoglycolipid by osmium-catalyzed periodate oxidation followed by treatment with mild alkali
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作者：Sen-Itiroh Hakomori
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1966
卷号：7
期号：6
页码：789-792
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The carbohydrate moiety of sphingoglycolipid, after preliminary acetylation, can be released by periodate oxidation catalyzed by a trace amount of osmium tetroxide, followed by alkaline treatment. Cerebroside, lactosyl ceramide, hematoside, globoside, and gangliosides were degraded to yield, respectively, galactose, lactose, sialyl lactose, a tetrasaccharide, and various oligosaccharides containing sialic acid. Oligosaccharides were separated by paper chromatography and paper electrophoresis. The procedure is useful for characterizing micromolar amounts of sphingoglycolipids.
关键词：osmium-catalyzed ; periodate ; oxidation ; acetylated ; sphingoglycolipid ; cleavage ; sphingosine-carbohydrate bond ; separation ; oligosaccharides