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标题：The intracellular distribution of sterols in Eurycotis floridana and its possible relation to subcellular membrane structures
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作者：N. L. Lasser ; R. B. Clayton
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1966
卷号：7
期号：3
页码：413-421
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The roach Eurycotis floridana was reared on a semisynthetic diet containing minimal cholesterol-4-¹⁴C (0.005%) supplemented with cholestanol-7α-³H (0.1%), and various tissues of the insect were separated into ``nuclear,'' ``mitochondrial,'' and ``microsomal'' fractions by differential centrifugation. Muscle and salivary gland were satisfactorily fractionated and results from these are reported together with some, as yet, incomplete results for nerve. The major concentrations of unesterified sterol and lipid phosphorus were in the mitochondrial and microsomal fractions. The unesterified sterols of the subcellular fractions were separated by alumina and gas-liquid chromatography. The emergent peaks corresponding to cholesterol, cholestanol, and Δ⁷-cholestenol (a metabolite of cholestanol) were assayed for ³H and ¹⁴C. No sterol was confined to any one subcellular fraction. The proportions of the three sterols were closely similar in all membranous components of the same tissue, but were different in different tissues. It is suggested that the different subcellular membranes of a given tissue contain similar repeating structural units in which the individual sterols occupy sterically characteristic spaces.
关键词：roach ; muscle ; salivary gland ; nerve ; fractionation ; sterols ; subcellular distribution ; membrane structure ; repeating unit