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  • 标题:Cerebroside galactosidase of brain
  • 本地全文:下载
  • 作者:Amiya K. Hajra ; David M. Bowen ; Yasuo Kishimoto
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1966
  • 卷号:7
  • 期号:3
  • 页码:379-386
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:The galactoside bond in cerebroside was found to be cleaved by an enzyme in rat and pig brain. Emulsified stearoyl-14C psychosine was used as the substrate and the extent of cleavage was studied by isolating and counting the stearoyl sphingosine (ceramide) formed. The reaction products, ceramide and galactose, were characterized by column and thin-layer chromatography. Cerebroside containing galactose-3H was also used to show liberation of galactose. Cholic acid was found to be required for activation of the enzyme, which has a pH optimum of 4.5. Similar cerebrosidase activity was found in spleen, kidney, and lung of rat; liver and heart showed very slight activity. The partially purified enzyme from pig brain also formed ceramide from ceramide lactoside, ceramide glucoside, and cerebronoyl psychosine. The enzyme was active toward o -nitrophenyl galactoside and could be fractionated by Sephadex chromatography into a fraction active toward the nitrophenyl galactoside only and a fraction active toward both this substrate and ceramide galactoside. Human spleen, normal and Gaucher, exhibited cerebrosidase activity.
  • 关键词:stearoyl-14C psychosine ; cerebronoyl-14C psychosine ; cerebrosidase ; rat organs ; pig brain ; ceramide ; ceramide lactoside ; galactosidase ; ceramide glucoside ; Gaucher spleen ; glucosidase
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