出版社:American Society for Biochemistry and Molecular Biology
摘要:The branched-chain fatty acids d -(+)-12-methyltetradecanoic acid (C15 anteiso) and d -(+)-14-methylhexadecanoic acid (C17, anteiso) were isolated from the lipids of Listeria monocytogenes and their 1- and 2-monoglycerides were prepared. Reaction intermediates and products were purified without isomerization by column chromatography. Thin-layer chromatography on Florisil impregnated with boric acid and nuclear magnetic resonance were used in characterizing the 1- and 2-monoglycerides. The value of the latter method for analyzing glyceride structure is discussed.